CosMc / BAC preps

The GSL is now offering automated services for extracting plasmid and BAC DNA from bacteria.

Using Agencourt’s bead chemistry technology, 96-well extractions are done quickly and consistently.

Depending on copy number and host strain, we typically extract 3-5µg of plasmid DNA per well and BAC DNA preps yield, on average, 0.5-2µg/well.

You inoculate the bacteria into half-well plates* (available at the GSL), we do the rest!

Paired with our gold-level sequencing or BAC finger-printing services, you can have your DNA and your data in the matter of a few days, no matter how busy you are.

_{*Bacteria are inoculated into 0.7ml 2xYT + appropriate antibiotic}

Image: Plasmid DNA (3µl) randomly picked from a 96-well plate and run on a 1% agarose gel. The gel image reveals our plasmid DNA extraction to be consistent and uniform.

DNA extractions

From fruit flies to mouse tails, using Agencourt’s DNAdvance genomic DNA extraction chemistry paired with our automated capabilities, 96 tissue samples are prepped in no time at all.

Ask us about our Drosophila genomic DNA extraction ½ reaction service (typically yields 1-2µg DNA per well) or ¼ reaction service (typically yields 0.5-1µg DNA per well) and get more bang for your buck!

While we do not currently offer 96-well plant genomic DNA extractions, we do have the capability. Interested? Please contact us for details and the possibility of adding this service.

Questions? Ask Jenn