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Qbot Print E-mail

Capacities: 3,500 colonies per hour for colony picking from media plates to appropriate well plates
Plate Consolidation 4 96-well plates to 1 384-well plate per minute
Filter spotting d 15 filters with 36,864 clones per filter in <3 hours
Microarrays 10,000 spots per field, 2 fields per microscope slide
210 slides per batch, batch time = <3 hours

ImagePicking, gridding, replicating, re-arraying and compressing or expanding libraries may be performed.

Picking
GSL sells two pieces of hardware required for the QBOT Picking set-up; QTrays and flat-bottom, Genetix Microtitre 96-well plates.

ImageQTrays are filled with user-created agar, grow colonies of E.coli, and fit the QBOT so these colonies may be picked off the agar. FILL a QTRAY EVENLY and EXACTLY with 200 mL of AGAR. This is the default picking height; any more or less agar may hinder or stop subsequent E. coli growth.

Genetix microtitre, 96-well plates are filled with the user-created agar and are inoculated by the QBot with colonies picked from the QTray. Seventy microliters per well is an ideal volume for these plates and may be added using the QFill.

Genetix Q-Fill
The Q-Fill may be used to fill Genetix 96- and 384-well plates with media for subsequent use with the Q-Bot or other applications.

 

Contact Jenn

Last Updated ( Sunday, 14 June 2009 )
 
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© 2008 The GENOMIC SCIENCES LABORATORY
Suite 2100 Partners II Bldg. | Centennial Campus | 840 Main Campus Drive | Raleigh, North Carolina, USA | (919) 513-3882 | Fax: (919) 513-3177
Dr. Jenn Schaff, Interim Director
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The GSL gratefully acknowledges the design/development support of the Center for the Biology of Nematode Parasitism.
Charles Opperman & David McK. Bird, directors. Mark Burke, BioInformatics, IT
Jim Gajan, site design & development.