454 Life Sciences Launches the GS FLX Titanium Series Reagents, Effectively Replacing Traditional Sanger Sequencing

454 Life Sciences Corporation, a Roche company, on October 1st 2008, announced the launch of the new GS FLX Titanium series reagents and software that are transforming the life science’s industry. The new reagent kits provide individual sequencing reads with an improved Q20 length of 400 base pairs (99% accuracy at the 400th base and higher for preceding bases) and a five-fold increase in throughput to 400 – 600 million base pairs per instrument run.

Flexibility

flx Address a broad range of applications with one versatile sequencing system for the fields of:

De novo Sequencing
Resequencing
Transcriptome Analysis
Gene Regulation Studies
Epigenetic Changes
Metagenomics & Microbial Diversity
Paleogenomics

Speed

LR70 format: Generate more than 100 million bases per 7.5-hour instrument run with an average yield of greater than 400,000 reads per run.

Accuracy

Achieve single-read accuracies that are greater than 99.5% over 200 bases per read, with reads averaging 200 to 300 bases, and obtain consensus accuracies of greater than 99.99%.

Productivity

Eliminate the laborious tasks of cloning and colony picking - generate libraries of whole genomes in a single-tube.

Cost efficiency

Reduce cost per raw base with rapid, efficient amplification and sequencing through picoliter format with massive parallelization. Benefit from flexible sample-loading options that support various throughput and experimental design needs, enabling efficient use of reagents and consumables.

Ease of use

Perform data analysis without the need for enterprise scale IT solutions. A Genome Sequencer FLX run that provides 100 million bases of high quality sequence data produces only 12 - 15 gigabytes of raw bioinformatics data which can easily be analysed and stored using an conventional IT infrastructure.

cDNA Synthesis protocol here

Thanks to Kevin Donohue (Entomology).

Typical Sample Requirements

You may download a powerpoint sample requirements presentation here

Sample Submission Form

Sample Submission form

Contact Jenn

Last Updated ( Tuesday, 02 June 2009 )